Views: 1 Author: 菌物健康 Publish Time: 2023-01-05 Origin: Site
Tetracyclic triterpenes and pentacyclic triterpenes in Ganoderma lucidum can enhance the body's anti-tumor immunity, and can kill tumor cells alone or in combination when they stop dividing. Ganoderma lucidum polysaccharides are composed of various monosaccharides, including glucose, galactose, mannose, arabinose, rhamnose, etc., and can be separated by paper chromatography. Ganoderma lucidum polysaccharides can make immune cells kill tumors in the early stage, or improve the formation ability of platelet fibrin to wrap early tumor masses and keep them in a dormant state for a long time.
In recent years, studies have confirmed that Ganoderma lucidum triterpenes and Ganoderma lucidum polysaccharides can inhibit malignant tumors such as liver cancer, lung cancer, cervical cancer, prostate cancer, breast cancer, and colon cancer. Today, let's take a look at its mechanism of action against colon cancer.
Anti-colon cancer effect and mechanism of Ganoderma lucidum triterpenoids
The research of LI et al. aims to evaluate the anticancer effect of neutral total triterpenes and acidic total triterpenes of Ganoderma lucidum in vivo and in vitro, and to explore their anticancer active ingredients on human colorectal cancer cells SW620. Adverse reactions to three kinds of human colon cancer cells and mouse embryonic fibroblasts were detected by thiazolium blue colorimetric method. The antitumor activity of two total triterpenes of Ganoderma lucidum on SW620 was detected in nude mice. The results of the study showed that the two total triterpenes of Ganoderma lucidum could reduce the viability of colorectal cancer cells in vitro, induce cancer cell apoptosis, and significantly inhibit the growth of colorectal cancer cells in mice during treatment, which is related to the pro-apoptotic protein cleaved Caspase-3 It is related to the upregulation of the level of cleaved Caspase-9. Therefore, its anticancer mechanism may be related to the activation of mitochondria-dependent pathways.
Anti-colon cancer effect and mechanism of Ganoderma lucidum polysaccharide
Related studies have observed the anti-colon cancer mechanism of enzymatically hydrolyzed Ganoderma lucidum polysaccharide (EGLP). The inhibitory effect of EGLP on the proliferation of human colorectal adenocarcinoma cells (HCT-116) was determined, and the results showed that EGLP inhibited the activity of HCT-116, but did not inhibit the activity of normal cells. Protein results showed that Bcl-2, which inhibited apoptosis, phosphorylated extracellular signal-regulated protein kinase (p-ERK) and phosphorylated protein kinase B1 (p-Akt1) proteins decreased in a dose-dependent manner with EGLP, while promoting Apoptotic protein Bax increased in a dose-dependent manner with EGLP. The mRNA results showed that the expression levels of Bcl-2 and COX-2 mRNA, which can inhibit apoptosis, decreased in a dose-dependent manner with EGLP. The expression levels of Bax and cleaved Caspase-3 mRNA, which promote apoptosis, increased in a dose-dependent manner with EGLP. It can be seen that EGLP may regulate p-Akt1/p-ERK signaling pathway and Bax/Bcl-2 protein expression to inhibit the growth of colon cancer cells.
